After the death of previous Boss, a gang war lasts for more than four years.
In Osaka during the Edo period, ruler Sanada Yukimura was in losing position, fearing Toyotomi's blood descendents would end, he impregnates one of five Shinano female ninja with the seed of Toyotomi Hideyoshi in hopes of continuing the bloodline. Princess Sen and the five female ninja escape from Osaka castle by blending in with the handmaidens. Lord Tokugawa Ieyasu who was supposed to rule all under the heavens was informed of this secret. So Ieyasu orders five samurai of the Iga Ninja clan (brought by Hattori Hanzo) to stop the Toyotomi bloodline by killing the Kunoichi ninja. An attack of ninja magic arts unfolds in a battle of the sexes.
When Shinsuke returns to Kyushu from the Russo-Japanese War he finds his Judo school hall neglected and in decay. His Judo teacher has died and his brother, Ryota, and the teacher's only daughter, Akiko, are greatly disturbed by Zanba, who intends to use the school for teaching the Ryukyu art of Karate.
A series of ghastly murders are being committed. The one similarity in each of the murders is that a strange buzzing sound is always heard right before the murder occurs. Is the killer invisible or possibly some other incredible creature?